Journal: International Journal of Molecular Sciences

Article Title: Proteomic Identification of Target Proteins of Thiodigalactoside in White Adipose Tissue from Diet-Induced Obese Rats

doi: 10.3390/ijms160714441

Figure Lengend Snippet: List of proteins showing differential expression between control and thiodigalactoside (TDG)-treated rats.

Article Snippet: The following antibodies were used in this study: anti-goat UCP-1, MDH2, PGM1, PPARγ, and CA3; anti-mouse CK and β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA); and anti-rabbit ANXA2 (Cell Signaling Technology, Beverly, MA, USA) and PGC-1α (Santa Cruz Biotechnology).

Techniques: Quantitative Proteomics, Control, Sequencing, Transduction, Membrane

Journal: Animal Nutrition

Article Title: Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks

doi: 10.1016/j.aninu.2022.03.008

Figure Lengend Snippet: Selected differentially expressed proteins in duck liver caused by pantothenic acid deficiency (PAD).

Article Snippet: Primary antibodies (1 μg/mL) against PGM1 (A6303; ABclonal), ME1 (A3956; ABclonal), IDH1 (A13245; ABclonal), and MDH2 (A13516; ABclonal) were used.

Techniques: Binding Assay, Phospho-proteomics

Journal: Animal Nutrition

Article Title: Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks

doi: 10.1016/j.aninu.2022.03.008

Figure Lengend Snippet: Integrated pathway analysis of liver proteins and metabolites in ducks significantly affected by pantothenic acid deficiency (PAD).

Article Snippet: Primary antibodies (1 μg/mL) against PGM1 (A6303; ABclonal), ME1 (A3956; ABclonal), IDH1 (A13245; ABclonal), and MDH2 (A13516; ABclonal) were used.

Techniques: Phospho-proteomics

Journal: Animal Nutrition

Article Title: Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks

doi: 10.1016/j.aninu.2022.03.008

Figure Lengend Snippet: Western blot analysis of phosphoglucomutase 1 (PGM1; A), malic enzyme 1 (ME1; B), isocitrate dehydrogenase (IDH1; C), and malate dehydrogenase 2 (MDH2; D) protein expression of liver tissue of ducks in the pantothenic acid deficient (PAD) and Control (CON) groups. Loading control, vinculin, was used to normalize the levels of PGM1, ME1, IDH1, and MDH2. Representative Western blots are shown. Values are means with their standard errors. a, b Mean values with unlike letters were significantly different ( P < 0.05). Data were analyzed by the Student's t -test.

Article Snippet: Primary antibodies (1 μg/mL) against PGM1 (A6303; ABclonal), ME1 (A3956; ABclonal), IDH1 (A13245; ABclonal), and MDH2 (A13516; ABclonal) were used.

Techniques: Western Blot, Expressing, Control

Journal: Animal Nutrition

Article Title: Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks

doi: 10.1016/j.aninu.2022.03.008

Figure Lengend Snippet: Disturbed metabolic pathways in liver tissue caused by pantothenic acid deficiency (PAD) based on proteomics and metabolomics profiles. Regulation is color and arrow coded in which red arrow ( Image 1 ) stands for upregulated, blue arrow ( Image 2 ) for downregulated in PAD group compared to control group. PYGL = alpha-1,4 glucan phosphorylase; PGM1 = phosphoglucomutase 1; FADH2 = Flavin adenine dinucleotide; TCA = tricarboxylic acid.

Article Snippet: Primary antibodies (1 μg/mL) against PGM1 (A6303; ABclonal), ME1 (A3956; ABclonal), IDH1 (A13245; ABclonal), and MDH2 (A13516; ABclonal) were used.

Techniques: Control